Pseudo-streaming potentials in Necturus gallbladder epithelium. II. The mechanism is a junctional diffusion potential.
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چکیده
منابع مشابه
Pseudo-streaming potentials in Necturus gallbladder epithelium. II. The mechanism is a junctional diffusion potential
The mechanisms of apparent streaming potentials elicited across Necturus gallbladder epithelium by addition or removal of sucrose from the apical bathing solution were studied by assessing the time courses of: (a) the change in transepithelial voltage (Vms). (b) the change in osmolality at the cell surface (estimated with a tetrabutylammonium [TBA+]-selective microelectrode, using TBA+ as a tra...
متن کاملPseudo-Streaming Potentials in Necturus Gallbladder Epithelium H. The Mechanism Is a Junctional Diffusion Potential
The mechanisms of apparent streaming potentials elicited across Necturus gallbladder epithelium by addition or removal of sucrose from the apical bathing solution were studied by assessing the time courses of: (a) the change in transepithelial voltage (Vm,), (b) the change in osmolality at the cell surface (estimated with a tetrabutylammonium [TBA+]-selective microelectrode, using TBA + as a tr...
متن کاملPseudo-streaming potentials in Necturus gallbladder epithelium. I. Paracellular origin of the transepithelial voltage changes
Apparent streaming potentials were elicited across Necturus gallbladder epithelium by addition or removal of sucrose from the apical bathing solution. In NaCl Ringer's solution, the transepithelial voltage (Vms) change (reference, basolateral solution) was positive with sucrose addition and negative with sucrose removal. Bilateral Cl- removal (cyclamate replacement) had no effect on the polarit...
متن کاملNecturus Gallbladder Epithelium
Transepithelial fluid transport(/,) and intracellular Na' and CIactivities (aNai , aCl;) were measured in isolated Necturus gallbladders to establish the contribution of different proposed apical membrane entry mechanisms to transepithelial salt transport . In 10 mM HC03-Ringer's, Jv was 13.5 ± 1 .1 ttl cm-'-h, 'and was significantly reduced by a low bicarbonate medium and by addition of amilor...
متن کاملOsmotic water permeability of Necturus gallbladder epithelium
An electrophysiological technique that is sensitive to small changes in cell water content and has good temporal resolution was used to determine the hydraulic permeability (Lp) of Necturus gallbladder epithelium. The epithelial cells were loaded with the impermeant cation tetramethylammonium (TMA+) by transient exposure to the pore-forming ionophore nystatin in the presence of bathing solution...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1992
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.99.3.317